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Can I read ON-Target Adverse Events of CRISPR-Cas9 Nuclease: More Chaotic than Expected on EtoBox?
ON-Target Adverse Events of CRISPR-Cas9 Nuclease: More Chaotic than Expected by Julian Boutin; David Cappellen; Juliette Rosier; Samuel Amintas; Sandrine Dabernat; Aurélie Bedel; François Moreau-Gaudry is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is ON-Target Adverse Events of CRISPR-Cas9 Nuclease: More Chaotic than Expected about?
CRISPR-Cas9 is a highly promising technology for clinical development. However, this powerful tool can induce adverse genomic events. The off-target genotoxicity is well described, predictable, detectable, and resolved by the use of new generations of Cas9 nucleases with high fidelity. In contrast, the ON-target genotoxicity due to a DNA double-strand break at the targeted locus is still underestimated. Here, we review several genomic outcomes induced by CRISPR-Cas9 from the insertion/deletion of a few bases to megabase-scale rearrangements. We hope to highlight this barely detectable complex safety concern to promote further studies to understand the mechanisms better, to detect these unwanted events, and to prevent them for the safety management of future CRISPR-Cas9 clinical trials.
Who reads ON-Target Adverse Events of CRISPR-Cas9 Nuclease: More Chaotic than Expected?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Julian Boutin; David Cappellen; Juliette Rosier; Samuel Amintas; Sandrine Dabernat; Aurélie Bedel; François Moreau-Gaudry
- Publisher
- Mary Ann Liebert Inc
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)