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Changes in Deoxyribonucleotide Biosynthesis During Carrot Somatic Embryogenesis by Claudio Stasolla; Natalia Loukanina; Hiroshi Ashihara; Edward C. Yeung; Trevor A. Thorpe is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Changes in Deoxyribonucleotide Biosynthesis During Carrot Somatic Embryogenesis about?

The activities of the de novo and salvage pathways of deoxynucleotide biosynthesis were investigated during the different stages of development in carrot (Daucus carota) somatic embryos by following the metabolic fate of [2-14 C]cytidine, which after conversion to CDP, is an intermediate of the de novo pathway of deoxypyrimidine nucleotides, and [2-14 C]deoxycytidine and [2-14 C]thymidine, substrates of the salvage pathway. Increasing de novo activity, estimated by cytidine (CR) incorporation into DNA, was observed as maturation progressed. This increase was parallel to a decrease in CR degradation to CO 2 . Utilization of CR for CMP synthesis was mainly due to the activity of cytidine kinase (CK), as low levels of non-specific phosphotransferases (NPT) measured with AMP as a phosphate donor and CR as a substrate, were detected throughout the course of the experiment. CR was also utilized for RNA synthesis, either directly, after conversion to CTP, or indirectly, after deamination to uridine and then converted to UTP. Salvage of supplied deoxycytidine (CdR) and thymidine (TdR) were also observed during embryo development. Turnover of CdR was a rapid event, as a large percentage of

Who reads Changes in Deoxyribonucleotide Biosynthesis During Carrot Somatic Embryogenesis?

Author

Claudio Stasolla; Natalia Loukanina; Hiroshi Ashihara; Edward C. Yeung; Trevor A. Thorpe

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0981-9428)

Published

2003

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)