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Assessment of heratibility of Alternaria helianthi resistance trait in sunflower using molecular markers by Murthy Uma; Lyngdoh Ian; Gopalakrishna T.; Shivanna M.B.; Prasad Theertha is a Agricultural and Biological Sciences article available to read on EtoBox.

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Leaf spot disease caused by Alternaria helianthi (Hansf.) is an important fungal disease of sunflower in India and elsewhere. Non-availability of known sources for resistance to Alternaria is a major constraint in sunflower breeding. Hence, seeds of sunflower genotypes were subjected to gamma-irradiation to develop mutant populations. Mutants were selected from the M 2 generation based on percent disease incidence and were passed on to the M 3 generation. Genomic DNA from leaf samples was evaluated by PCR amplification using random decamer primers and the amplified DNA fragments from mutants were compared with their respective controls. Single marker and stepwise regression analysis carried out in relation to percent disease index indicate that the alleles OPC5-B, K, J, OPA12-D and OPA15-A are strongly associated with Alternaria helianthi resistance.

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Author

Murthy Uma; Lyngdoh Ian; Gopalakrishna T.; Shivanna M.B.; Prasad Theertha

Publisher

National Library of Serbia

Published

2005

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)