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Quantification of astrocyte volume changes during ischemia in situ reveals two populations of astrocytes in the cortex of GFAP/EGFP mice by Jana Benesova; Miroslav Hock; Olena Butenko; Iva Prajerova; Miroslava Anderova; Alexander Chvatal is a Neuroscience article available to read on EtoBox.
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## Abstract Energy depletion during ischemia leads to disturbed ionic homeostasis and accumulation of neuroactive substances in the extracellular space, subsequently leading to volume changes in astrocytes. Confocal microscopy combined with 3D reconstruction was used to quantify ischemia‐induced astrocyte volume changes in cortical slices of GFAP/EGFP transgenic mice. Twenty‐minutes of oxygen‐glucose deprivation (OGD) or oxygen‐glucose deprivation combined with acidification (OGD~pH 6.8~) revealed the presence of two distinct astrocytic populations, the first showing a large volume increase (HR astrocytes) and the second displaying a small volume increase (LR astrocytes). In addition, changes in resting membrane potential (V~m~), measured by the patch‐clamp technique, supported the existence of two astrocytic populations responding differently to ischemia. Although one group markedly depolarized during OGD or OGD~pH 6.8~, only small changes in V~m~ toward more negative values were observed in the second group. Conversely, acidification (ACF~pH 6.8~) led to a uniform volume decrease in all astrocytes, accompanied by only a small depolarization. Interestingly, two differently respond
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- Author
- Jana Benesova; Miroslav Hock; Olena Butenko; Iva Prajerova; Miroslava Anderova; Alexander Chvatal
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)
- Published
- 2008
- Language
- EN
- Field
- Neuroscience (Life Sciences)