About this document
TLC Retention Factor Calculation Guide by hamid is a document available to read on EtoBox.
Calculating Retention Factors (Rf) for Thin Layer Chromatography involves measuring the distance from the starting point to the center of a spotted substance (distance a) and the distance from the starting point to the solvent front (distance b). The Rf value is calculated by dividing distance a by distance b and represents the position of the substance on the TLC plate, with values ranging from 0 to 1. An Rf value is used to identify substances separated via thin layer chromatography.
- Author
- hamid
- Language
- EN