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Expression of ribosome modulation factor (RMF) in Escherichia coli requires ppGpp by Kaori Izutsu; Akira Wada; Chieko Wada is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Expression of ribosome modulation factor (RMF) in Escherichia coli requires ppGpp about?

**Background** During the transition from the logarithmic to the stationary phase, 70S ribosomes are dimerized into the 100S form, which has no translational activity. Ribosome Modulation Factor (RMF) is induced during the stationary phase and binds to the 50S ribosomal subunit, which directs the dimerization of 70S ribosomes. Unlike many other genes induced in the stationary phase, __rmf__ transcription is independent of the sigma S. To identify the factors that regulate the growth phase‐dependent induction of __rmf__, mutant strains deficient in global regulators were examined for __lacZ__ expression directed by the __rmf__ promoter. **Results** Among mutants of defective global regulators, only ppGpp deficiency (__relA‐spoT__ double mutant) drastically reduced the level of __rmf__ transcription to less than 10% of that seen in the wild‐type. Neither RMF nor 100S ribosomes were detected in this mutant in the stationary phase. __rmf__ transcription correlated well with cellular ppGpp levels during amino acid starvation, IPTG induction of P__trc‐relA455__ and in other mutants with artificially increased ppGpp levels. Although the growth rate also correlated inversely with both ppGp

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Author

Kaori Izutsu; Akira Wada; Chieko Wada

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1356-9597)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)