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The Method of Intracellular Fluorescent Species in Situ Production Within Living Cells by Femtosecond Laser Pulses by Osychenko, A A; Zalessky, A D; Shakhov, A M; Astafiev, A A; Syrchina, M S; Tochilo, U A; Karmenyan, A V; Nadtochenko, V A is a Physics and Astronomy article available to read on EtoBox.
What is The Method of Intracellular Fluorescent Species in Situ Production Within Living Cells by Femtosecond Laser Pulses about?
## Abstract Here we report on a novel non-invasive technique of __in situ__ fluorescence species creation with the use of tightly-focused femtosecond laser beam with 800 nm wavelength. Fluorescent species can be created in a strictly defined area inside a living cell, without damaging the plasma membrane and surrounding structures. Species are characterized by bright fluorescence with a maximum at 520 nm, high stability and low toxicity. No significant effect of fluorescent species on cell viability and development is observed. The technique of __in situ__ fluorescence species creation is an attractive tool for __in vivo__ cell visualization and intracellular tracking because of its high accuracy and low damage effect.
Who reads The Method of Intracellular Fluorescent Species in Situ Production Within Living Cells by Femtosecond Laser Pulses?
It is typically read by researchers, students, and practitioners in Physics and Astronomy.
- Author
- Osychenko, A A; Zalessky, A D; Shakhov, A M; Astafiev, A A; Syrchina, M S; Tochilo, U A; Karmenyan, A V; Nadtochenko, V A
- Publisher
- Institute of Physics; IOP Publishing (ISSN 1742-6588)
- Published
- 2019
- Language
- EN
- Field
- Physics and Astronomy (Physical Sciences)