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SARS-CoV-2 S and N protein peptides drive invasion abilities of colon cancer cells through TGF-β1 regulation 1 1 Introduction 1 2 Materials and methods 2 2.1 Cell lines 2 2.2 SARS-CoV-2 proteins and cells treatment 2 2.3 Conditioned medium (CM) preparation 2 2.4 Western blotting 2 2.5 Transwell migration assay 2 2.6 Invasion assay 3 2.7 MMP-7 and TGF-β1 ELISA assays 3 2.8 siRNA transfection 3 2.9 Statistical analysis 3 3 Results 3 3.1 SARS-CoV-2 S and N protein peptides induce EMT in colon cancer cells 3 3.2 SARS-CoV-2 S and N protein peptides drive invasive properties of colon cancer cells 4 3.3 SARS-CoV-2 S and N protein peptides stimulate colon cancer cells to TGF-β1 secretion 5 3.4 TGF-β1 neutralization abrogates the SARS-CoV-2-dependent EMT induction and invasion abilities in colon cancer cells 5 3.5 TGFβR downregulation revokes the EMT induction and invasion abilities in colon cancer cells treated with SARS-CoV-2 pro ... 5 3.6 Inhibition of the TGF -β1-dependent pathways impede the SARS-CoV-2 stimulatory effect 5 3.7 SARS-CoV-2 proteins induced a bystander effect in colon cancer cells 7 4 Discussion 10 Funding 11 CRediT authorship contribution statement 11 Declaration of c

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nonfiction