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Vesicle Disruption, Plasma Membrane Bleb Formation, and Acute Cell Death Caused by Illumination with Blue Light in Acridine Orange-loaded Malignant Melanoma Cells by Hiromi Hiruma; Takashi Katakura; Tamie Takenami; Satoshi Igawa; Maho Kanoh; Takao Fujimura; Tadashi Kawakami is a Medicine article available to read on EtoBox.

What is Vesicle Disruption, Plasma Membrane Bleb Formation, and Acute Cell Death Caused by Illumination with Blue Light in Acridine Orange-loaded Malignant Melanoma Cells about?

Acridine orange (AO), a weakly basic fluorescent dye, is permeable to plasma and vesicle membranes and preferentially remains in intracellular acidic regions. Using fluorescence microscopy, we observed dynamic changes in AO-loaded cultured malignant melanoma cells during illumination with blue light. Immediately after the start of the illumination, the successive disruption of vesicles was observed as a flash of fluorescence, and shortly after that, blebs were formed on the plasma membrane. These cells died within 5 min. Vesicle disruption was completely inhibited when cells were treated with the vacuolar H(+)-ATPase inhibitor bafilomycin A1 followed by loading with AO, but not when bafilomycin A1 was treated after AO loading. Thus, the filling of AO in the vesicle, which is driven by vacuolar H(+)-ATPase, is initially required for vesicle disruption. In contrast, bafilomycin A1 did not prevent plasma membrane blebbing, indicating that the blebs are formed independently of the vesicle disruption. Acute cell death was inhibited by treatment with bafilomycin A1 before but not after AO loading. Thus, AO- and blue light-induced acute cell death is associated with vesicle disruption rat

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Author

Hiromi Hiruma; Takashi Katakura; Tamie Takenami; Satoshi Igawa; Maho Kanoh; Takao Fujimura; Tadashi Kawakami

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1011-1344)

Published

2007

Language

EN

Field

Medicine (Physical Sciences)