Can I read Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes on EtoBox?

Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes by Salvatore A. E. Marras is a book available to read on EtoBox.

What is Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes about?

With the introduction of simple and relatively inexpensive methods for labeling nucleic acids with nonradioactive labels, doors have been opened that enable nucleic acid hybridization probes to be used for research and development, as well as for clinical diagnostic applications. The use of fluorescent hybridization probes that generate a fluorescence signal only when they bind to their target enables real-time monitoring of nucleic acid amplification assays. The use of hybridization probes that bind to the amplification products in real-time markedly improves the ability to obtain quantitative results. Furthermore, real-time nucleic acid amplification assays can be carried out in sealed tubes, eliminating carryover contamination. Because fluorescent hybridization probes are available in a wide range of colors, multiple hybridization probes, each designed for the detection of a different nucleic acid sequence and each labeled with a differently colored fluorophore, can be added to the same nucleic acid amplification reaction, enabling the development of high-throughput multiplex assays. It is therefore important to carefully select the labels of hybridization probes, based on the t

Author

Salvatore A. E. Marras

Language

EN