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CCK regulates osteogenic differentiation through TNFα/NF-κB in peri-implantitis by LongHang Chou; YaTing Chang; KaiWen Lan; Meng Liu; YuKun Lu; XiaoLei Li; PeiRu Li; Yue Xu is a Medicine article available to read on EtoBox.
What is CCK regulates osteogenic differentiation through TNFα/NF-κB in peri-implantitis about?
## Objective Peri-implantitis is characterized by peri-implant mucositis and alveolar bone resorption. This study investigated cholecystokinin (__CCK__) expression and the mechanism underlying its involvement in peri-implantitis. ## Methods mRNA sequencing was performed using the Gene Expression Omnibus database GSE106090. Human bone marrow mesenchymal stem cells (hBMSCs) were pretreated with various concentrations of CCK (0, 10, 30, or 100 nM) for 1 hour before induction in osteogenic differentiation medium for 2 weeks. Alkaline phosphatase (ALP) activity was determined, and the cells were stained with alizarin red. The expression levels of __TNFα__ and the osteogenic markers __ALP__, __RUNX2__, and __OCN__ were measured using quantitative real-time PCR. TNFα, phosphorylated P65, and total P65 levels were determined by western blot. ## Results Compared with healthy individuals, 262 and 215 genes were up- and down-regulated, respectively, in the periodontal tissues of patients with peri-implantitis. __CCK__ expression was significantly upregulated in patients with peri-implantitis. CCK reduced ALP activity, osteogenic differentiation, and levels of the osteogenic markers __ALP__, _
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- Author
- LongHang Chou; YaTing Chang; KaiWen Lan; Meng Liu; YuKun Lu; XiaoLei Li; PeiRu Li; Yue Xu
- Publisher
- SAGE Publications
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)