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Comparison of expression systems in the yeastsSaccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe andYarrowia lipolytica. Cloning of two novel promoters fromYarrowia lipolytica by Sven Müller; Thomas Sandal; Peter Kamp-Hansen; Henrik Dalbøge is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Comparison of expression systems in the yeastsSaccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe andYarrowia lipolytica. Cloning of two novel promoters fromYarrowia lipolytica about?

We have compared expression systems based on autonomously replicating vectors in the yeasts Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Hansenula polymorpha and Yarrowia lipolytica in order to identify a more suitable host organism for use in the expression cloning method (Dalbøge and Heldt-Hansen, 1994) in which S. cerevisiae has traditionally been used. The capacity of the expression systems to secrete active forms of six fungal genes encoding the enzymes galactanase, lipase, polygalacturonase, xylanase and two cellulases was examined, as well as glycosylation pattern, plasmid stability and transformation frequency. All of the examined alternative hosts were able to secrete more active enzyme than S. cerevisiae but the relative expression capacity of the individual hosts varied significantly in a gene-dependent manner. One of the most attractive of the alternative host organisms, Y. lipolytica, yielded an increase which ranged from 4•5 times to more than two orders of magnitude. As the initially employed Y. lipolytica XPR2 promoter is unfit in the context of expression cloning, two novel promoter sequences for highly expressed genes present in only

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Author

Sven Müller; Thomas Sandal; Peter Kamp-Hansen; Henrik Dalbøge

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)