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Geotrichum candidum acetophenone reductase immobilization on reduced graphene oxide: A promising biocatalyst for green asym ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents and materials 2 2.2 GO preparation 3 2.3 rGO preparation 3 2.4 GcAPRD immobilization 3 2.5 Measurement of enzyme activity 3 2.6 Recyclability of GO-GcAPRD and rGO-GcAPRD 3 2.7 Micromole scale asymmetric reduction of ketones by rGO-GcAPRD 3 2.8 Millimole scale asymmetric reduction of ketones by rGO-GcAPRD 4 2.9 Characterization of GO, rGO, GO-GcAPRD, and rGO-GcAPRD by SEM, EDX, FTIR, XRD, and TG/DTA 4 3 Results and discussions 4 3.1 GcAPRD immobilization on GO and rGO 4 3.2 Functional group analysis by FTIR 5 3.3 XRD analysis 5 3.4 Thermogravimetric (TG) analysis 6 3.5 Morphology study 6 3.6 Temperature profiles of GO-GcAPRD and rGO-GcAPRD 6 3.7 Recyclability 6 3.8 Substrate specificity of GO-GcAPRD and rGO-GcAPRD 6 4 Conclusion 7 CRediT authorship contribution statement 8 Declaration of Competing Interest 8 Acknowledgments 8 Appendix A Supporting information 8 References 8

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nonfiction