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Reducing oxidative stress by κ-carrageenan and C60HyFn: The post-thaw quality and antioxidant status of Azari water buffalo bull semen by N. Ramazani; F. Mahd Gharebagh; A. Soleimanzadeh; H.O. Arslan; E. Keles; D.G. Gradinarska-Yanakieva; D. Arslan-Acaröz; M. Zhandi; A. Baran; E. Ayen; D.A. Dinç is a Medicine article available to read on EtoBox.

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Azeri water buffalo is a species of great interest due to the high quality of its products such as milk. Due to the decreasing trend of its number and risk of extinction in the future, our attention is directed towards ensuring the preservation of its genetic reserves by keeping its sperm. Using antioxidants in semen extender is one of the ways to reduce the detrimental effects of freezing process on post-thawed quality of spermatozoa. This study was conducted to determine the effect of κ-carrageenan (k-CRG) and C60HyFn supplemented semen extender on the quality of post-thawed Azari water buffalo spermatozoa. A total of 30 semen samples were obtained from three buffaloes using an artificial vagina (twice a week for five weeks = 10 replicates). The samples (n = 3) from each replicate were pooled and divided into equal aliquots to prepare 14 extender groups, including control (C), k-0.2, K-0.4, K-0.6, K-0.8 (containing 0.2, 0.4, 0.8 mg K-CRG/mL, respectively), C-0.1, C-0.2, C-0.4, C-0.8, C-1, C-5, C-10, C-20, and C-40 (containing 0.1, 0.2, 0.4, 0.6, 0.8, 1, 5, 10, 20, 40 μM C60HyFn, respectively), and then frozen. After thawing, motility and velocity parameters, plasma membrane integ

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Author

N. Ramazani; F. Mahd Gharebagh; A. Soleimanzadeh; H.O. Arslan; E. Keles; D.G. Gradinarska-Yanakieva; D. Arslan-Acaröz; M. Zhandi; A. Baran; E. Ayen; D.A. Dinç

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)