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Expression of Ca2+ channel subunits during cardiac ontogeny in mice and rats: Identification of fetal α1C and β subunit isoforms by Hannelore Haase; Birgit Pfitzmaier; Maureen W. McEnery; Ingo Morano is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression of Ca2+ channel subunits during cardiac ontogeny in mice and rats: Identification of fetal α1C and β subunit isoforms about?
Functional cardiac L-type calcium channels are composed of the pore-forming ␣ 1C subunit and the regulatory  2 and ␣ 2 /␦ subunits. To investigate possible developmental changes in calcium channel composition, we examined the temporal expression pattern of ␣ 1C and  2 subunits during cardiac ontogeny in mice and rats, using sequence-specific antibodies. Fetal and neonatal hearts showed two size forms of ␣ 1C with 250 and 220 kDa. Quantitative immunoblotting revealed that the rat cardiac 250-kDa ␣ 1C subunit increased about 10-fold from fetal days 12-20 and declined during postnatal maturation, while the 220-kDa ␣ 1C decreased to undetectable levels. The expression profile of the 85-kDa  2 subunit was completely different:  2 was not detected at fetal day 12, rose in the neonatal stage, and persisted during maturation. Additional  2 -stained bands of 100 and 90 kDa were detected in fetal and newborn hearts, suggesting the transient expression of  2 subunit variants. Furthermore, two fetal proteins with  4 immunoreactivity were identified in rat hearts that declined during prenatal development. In the fetal rat heart,  4 gene expression was confirmed by RT-PCR. Cardiac and br
Who reads Expression of Ca2+ channel subunits during cardiac ontogeny in mice and rats: Identification of fetal α1C and β subunit isoforms?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hannelore Haase; Birgit Pfitzmaier; Maureen W. McEnery; Ingo Morano
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)