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The Organic Osmolytes Betaine and Proline Are Transported by a Shared System in Early Preimplantation Mouse Embryos by Mohamed-Kheir Idris Anas; Mary-Anne Hammer; Michael Lever; Jo-Ann L. Stanton; Jay M. Baltz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The Organic Osmolytes Betaine and Proline Are Transported by a Shared System in Early Preimplantation Mouse Embryos about?
## Abstract Betaine and proline protect preimplantation mouse embryos against increased osmolarity and decreased cell volume, implying that they may function as organic osmolytes. However, the transport system(s) that mediates their accumulation in fertilized eggs and early embryos was unknown, and previously identified mammalian organic osmolyte transporters could not account for their transport. Here, we report that there is a single saturable transport component shared by betaine and proline in 1‐cell mouse embryos. A series of inhibitors had nearly identical effects on both betaine and proline transport by this system. In addition, K~i~ values for reciprocal inhibition of betaine and proline transport were ∼100–300 μM, similar to K~m~ values (∼200–300 μM) for their transport, and both had similar maximal transport rates (V~max~). The K~i~ values for inhibition of betaine and proline transport by dimethylglycine were similar (∼2 mM), further supporting transport of both substrates by a single transport system. Finally, betaine and proline transport each required Na^+^‐ and Cl^−^. These data were consistent with a single, Na^+^‐ and Cl^−^‐requiring, betaine/proline transport syst
Who reads The Organic Osmolytes Betaine and Proline Are Transported by a Shared System in Early Preimplantation Mouse Embryos?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mohamed-Kheir Idris Anas; Mary-Anne Hammer; Michael Lever; Jo-Ann L. Stanton; Jay M. Baltz
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)