About this Biochemistry, Genetics and Molecular Biology article
Palmitate Decreases Proton Pumping of Liver-type Cytochrome C Oxidase by Icksoo Lee; Bernhard Kadenbach is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
The H^+^/e^–^ stoichiometry of reconstituted cytochrome __c__ oxidase from bovine kidney, containing subunit VIaL (liver type), is 0.5 under standard conditions but 1.0 on addition of 1% cardiolipin to the lipid mixture (asolectin). Low concentrations of palmitate (half‐maximal effect at 0.5 μm), but not laurate, myristate, stearate, oleate, 1‐hexadecanol, palmitoyl glycerol and palmitoyl CoA, decreased the H^+^/e^–^ ratio in the presence of cardiolipin from 1.0 to 0.5, accompanied by an increase of coupled, but not of uncoupled respiration of proteoliposomes. Cardiolipin and palmitate did not influence the H^+^/e^–^ stoichiometry and respiration of reconstituted cytochrome __c__ oxidase from bovine heart, containing subunit VIaH (heart‐type). The H^+^/e^–^ stoichiometry of the heart enzyme, however, is decreased from 1.0 to 0.5 by 5 mm intraliposomal ATP (instead of 5 mm ADP). It is assumed that palmitate binds to subunit VIaL. The partial uncoupling of proton pumping in cytochrome __c__ oxidase is suggested to participate in mammalian thermogenesis.
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- Author
- Icksoo Lee; Bernhard Kadenbach
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)