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Can I read miR-99a in bone homeostasis: Regulating osteogenic lineage commitment and osteoclast differentiation on EtoBox?
miR-99a in bone homeostasis: Regulating osteogenic lineage commitment and osteoclast differentiation by Moura, Sara Reis; Bras, Joao Paulo; Freitas, Jaime; Osório, Hugo; Barbosa, Mario Adolfo; Santos, Susana Gomes; Almeida, Maria Ines is a Medicine article available to read on EtoBox.
What is miR-99a in bone homeostasis: Regulating osteogenic lineage commitment and osteoclast differentiation about?
## Background: The tight coupling between osteoblasts and osteoclasts is essential to maintain bone homeostasis. deregulation of this process leads to loss and deterioration of the bone tissue causing diseases, such as osteoporosis. micrornas are able to control bone-related mechanisms and have been explored as therapeutic tools. in this study, we investigated the potential of mir-99a-5p to modulate osteogenic differentiation, osteoclastogenesis, and the osteoblasts-osteoclasts crosstalk. ## Methods: To achieve this goal, human primary mesenchymal stem/stromal cells (msc) were differentiated into osteoblasts and adipocytes, and mir-99a-5p expression was evaluated by rt-qpcr. knockdown and overexpression experiments were conducted to modulate mir-99a-5p expression in mc3t3 cells. cell proliferation and cell death/apoptosis were evaluated by resazurin assay and flow cytometry, respectively. proteomic analysis was used to identify the mir-99a-5p regulatory network, and elisa to evaluate opg levels in the cell culture supernatant. conditioned media from mc3t3-transfected cells was used to culture raw 264.7 cells and the effect on osteoclast differentiation was assessed. human primary m
Who reads miR-99a in bone homeostasis: Regulating osteogenic lineage commitment and osteoclast differentiation?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Moura, Sara Reis; Bras, Joao Paulo; Freitas, Jaime; Osório, Hugo; Barbosa, Mario Adolfo; Santos, Susana Gomes; Almeida, Maria Ines
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 8756-3282)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)