Can I read Quantitative Analysis of Phosphatidylcholine in Rat Liver Tissue by Nanoflow Liquid Chromatography/tandem Mass Spectrometry on EtoBox?

Quantitative Analysis of Phosphatidylcholine in Rat Liver Tissue by Nanoflow Liquid Chromatography/tandem Mass Spectrometry by Eun Jeong Ahn; Hanna Kim; Bong Chul Chung; Myeong Hee Moon is a Chemistry article available to read on EtoBox.

What is Quantitative Analysis of Phosphatidylcholine in Rat Liver Tissue by Nanoflow Liquid Chromatography/tandem Mass Spectrometry about?

## Abstract A quantitative method was developed for the determination of phosphatidylcholine (PC) species concentration using nanoflow LC‐ESI‐MS/MS. In this study, a calibration method is developed to determine the effect of PC carbon chain length on MS peak intensity. Using the multiple standard addition method, a relationship between the peak intensities of different PC species from nanoflow LC‐MS and carbon chain length is established first using different injection amounts of PC standards. From this relationship, a calibration curve for each carbon chain length can be obtained for the concentration calculation. It was found that the MS peak area of PC species analyzed by nanoflow LC‐MS linearly decreased with increased acyl carbon numbers, and that the effect of the degree of acyl chain unsaturation on MS peak intensity was minimized when the injection amount was maintained at less than 1 pmol. The method was applied for the quantitative calculation of 34 PC species from rat liver, which were identified from data‐dependent MS/MS analysis during nanoflow LC separation.

Who reads Quantitative Analysis of Phosphatidylcholine in Rat Liver Tissue by Nanoflow Liquid Chromatography/tandem Mass Spectrometry?

Author

Eun Jeong Ahn; Hanna Kim; Bong Chul Chung; Myeong Hee Moon

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1615-9306)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)