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Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control by Chenyu Su; Shanshan Liu; Meixue Sun; Qianlong Yu; Changyou Li; Robert I. Graham; Xiufang Wang; Xinwei Wang; Pengjun Xu; Guangwei Ren is a Engineering article available to read on EtoBox.
What is Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control about?
The application of RNA interference (RNAi) technology for pest control is environmentally friendly and accurate. However, the efficiency of RNAi is often inconsistent and unreliable, and finding a suitable carrier element is considered critical to success in overcoming biotic and abiotic barriers to reach the target site. The fall armyworm, Spodoptera frugiperda (FAW), which is one of most important global agricultural pests, has recently spread rapidly to other parts of the world. In this study, a method to improve the stability and RNAi efficiency of the dsRNA carrier complex was reported. Methoprene-tolerant gene (Met) was selected as a target, a gene which is critical to the growth and development of FAW. Biomaterials nanoliposomes (LNPs) were modified with polyethylenimine (PEI) to deliver the dsRNA of Met. The synthesized Met3@PEI@LNPs reached a size of 385 nm and were found to load dsRNA effectively. Through stability and protection assays, it was found that LNPs provided reliable protection. In addition, the release curve also demonstrated that LNPs were able to prevent premature release under alkaline condition of the insect midgut but accelerate the release after entering
Who reads Delivery of Methoprene-Tolerant dsRNA to Improve RNAi Efficiency by Modified Liposomes for Pest Control?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Chenyu Su; Shanshan Liu; Meixue Sun; Qianlong Yu; Changyou Li; Robert I. Graham; Xiufang Wang; Xinwei Wang; Pengjun Xu; Guangwei Ren
- Publisher
- American Chemical Society (ACS)
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)