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Direct Observation of the Distribution of Fluorescent Probes in Phosphatidylcholine/cholesterol Vesicles Using Flow Microfluorometry by Janet K. Allen; David K. Dennison; Kenneth S. Schmitz; Joel D. Morrisett is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Direct Observation of the Distribution of Fluorescent Probes in Phosphatidylcholine/cholesterol Vesicles Using Flow Microfluorometry about?

The technique of flow microfluorometry has been extended to the study of small lipid complexes to assess either the lipid (hydrophobic) or aqueous (hydrophilic) compartments of selected natural or model membrane systems. sn-1-Palmitoyl-sn-2-oleoyl-phosphatidylcholine/cholesterol unilamellar vesicles, averaging 268 nm in diameter and containing varying concentrations of the synthetic lipophile probe, sn-1-palmitoyl-sn-2-12-[N-4-nitrobenzo-2-oxa-1,3- diazole]-aminocaproyl-phosphatidylcholine (NBD-PC), were analyzed using an Ortho Series 50-H Cytofluorograf and an Ortho 2150 computer system. NBD-labeled vesicles were analyzed for green fluorescence and the intensity of scattered light, the later being analyzed both at low angle (2-5 degrees) and at 90 degrees to the incident beam. At the high amplification required for vesicle detection, background signals from the sheath buffer, nonspecific laser light, and electronic noise were observed. However, this background noise signal was removed by appropriately setting a discriminator window. Profiles of signals falling within this region were then constructed. For the settings selected, more than 98% of data recorded could be attributed to

Who reads Direct Observation of the Distribution of Fluorescent Probes in Phosphatidylcholine/cholesterol Vesicles Using Flow Microfluorometry?

Author

Janet K. Allen; David K. Dennison; Kenneth S. Schmitz; Joel D. Morrisett

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1984

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)