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Gastruloid Development Competence Discriminates Different States of Pluripotency by Federica Cermola; Cristina D'Aniello; Rosarita Tatè; Dario De Cesare; Alfonso Martinez-Arias; Gabriella Minchiotti; Eduardo Jorge Patriarca is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Gastruloid Development Competence Discriminates Different States of Pluripotency about?
## Summary Floating spheroidal aggregates of mouse embryonic stem cells can develop into polarized/elongated organoids, namely gastruloids. We set up a high-performing assay to measure gastruloid formation efficiency (GFE), and found that GFE decreases as pluripotency progresses from naive (GFE ≥ 95%) to primed (GFE = 0) state. Specifically, we show that primed EpiSCs fail to generate proper cell aggregates, while early-primed EpiLCs aggregate but eventually fail to develop into elongated gastruloids. Moreover, we characterized proline-induced cells (PiCs), a LIF-dependent reversible early-primed state of pluripotency, and show that PiCs are able to generate gastruloids (GFE ∼ 50%) and are also competent to differentiate into primordial germ cell-like cells. Thus, we propose the GFE assay as a valuable functional tool to discriminate different states of the pluripotency continuum.
Who reads Gastruloid Development Competence Discriminates Different States of Pluripotency?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Federica Cermola; Cristina D'Aniello; Rosarita Tatè; Dario De Cesare; Alfonso Martinez-Arias; Gabriella Minchiotti; Eduardo Jorge Patriarca
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)