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Identification of a sperm protein 17 CTL epitope restricted by HLA-A1 by Maurizio Chiriva-Internati; Zhiqing Wang; Stephanie Pochopien; Emanuela Salati; Seah H. Lim is a Medicine article available to read on EtoBox.
What is Identification of a sperm protein 17 CTL epitope restricted by HLA-A1 about?
## Abstract Sperm protein 17 (Sp17) is a novel cancer‐testis antigen. We previously reported the successful generation of Sp17‐specific HLA‐A1‐restricted cytotoxic T‐lymphocytes (CTLs) from the peripheral blood of a healthy donor using a recombinant Sp17 protein. These CTLs were able to kill not only target cells pulsed with the recombinant protein but also fresh Sp17^+^ tumor cells. In the present study, we have identified a nonapeptide sequence within the Sp17 protein that is predicted to have a high binding affinity for the HLA‐A1 molecules. We generated the synthetic nonapeptide and successfully propagated a peptide‐specific CTL line. We confirmed that peptide Sp17~103–111~ (ILDSSEEDK) contains an Sp17 CTL epitope restricted by HLA‐A1 and identified amino acid residues 104, 107, 109 and 110 as crucial for the CTL lysis. Our findings therefore provide the tool for the characterization of CD8 T‐cell function __in vivo__ and generation of epitope‐specific treatment strategies. © 2003 Wiley‐Liss, Inc.
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- Author
- Maurizio Chiriva-Internati; Zhiqing Wang; Stephanie Pochopien; Emanuela Salati; Seah H. Lim
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)