Opening book details…
Can I read Quantitative Determination of Diazepam, Nitrazepam and Flunitrazepam in Tablets Using Thin-layer Chromatography–densitometry Technique on EtoBox?
Quantitative Determination of Diazepam, Nitrazepam and Flunitrazepam in Tablets Using Thin-layer Chromatography–densitometry Technique by Mehdi Bakavoli; Massoud Kaykhaii is a Chemistry article available to read on EtoBox.
What is Quantitative Determination of Diazepam, Nitrazepam and Flunitrazepam in Tablets Using Thin-layer Chromatography–densitometry Technique about?
A simple and reliable assay for diazepam, nitrazepam and flunitrazepam in tablets by high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)-densitometry is described. A quantity of a ground tablet mass, equal to the average weight of one tablet was sonicated in MeOH, mixed with appropriate internal standard, filtered and either injected directly into the liquid chromatograph, or after evaporation and reconstitution of an aliquot of the extract, was spotted on a silica gel thin-layer plate. A variable UV detector, operated at 254 nm was employed in both procedures. A C18, reversed phase 7 microm column was used for HPLC analysis; the mobile phase was a 1:1 (v/v) mixture of MeOH (40 degrees C) and 0.01 M phosphate buffer (pH 7, 80 degrees C). The TLC plate was developed in an unsaturated chromatographic chamber containing 100 ml chloroform-acetone (9:1); at room temperature, the mobile phase was allowed to travel 15 cm. The percentage of the active ingredient content of each tablet obtained by both procedures, was in the range of the stated amount except for one brand of diazepam tablets which contained approximately 23% less active ingredient than the mini
Who reads Quantitative Determination of Diazepam, Nitrazepam and Flunitrazepam in Tablets Using Thin-layer Chromatography–densitometry Technique?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Mehdi Bakavoli; Massoud Kaykhaii
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)