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Can I read A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media on EtoBox?
A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media by Berny, Clément; Le Fà̈vre, Raphael; Guyot, François; Blondeau, Karine; Guizonne, Christine; Rousseau, Emilie; Bayan, Nicolas; Alphandéry, Edouard is a Engineering article available to read on EtoBox.
What is A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media about?
We report the synthesis in large quantity of highly pure magnetosomes for medical applications. For that, magnetosomes are produced by MSR-1 __Magnetospirillum gryphiswaldense__ magnetotactic bacteria using minimal growth media devoid of uncharacterized and toxic products prohibited by pharmaceutical regulation, i.e., yeast extract, heavy metals different from iron, and carcinogenic, mutagenic and reprotoxic agents. This method follows two steps, during which bacteria are first pre-amplified without producing magnetosomes and are then fed with an iron source to synthesize magnetosomes, yielding, after 50 h of growth, an equivalent OD~565~ of ~8 and 10 mg of magnetosomes in iron per liter of growth media. Compared with magnetosomes produced in non-minimal growth media, those particles have lower concentrations in metals other than iron. Very significant reduction or disappearance in magnetosome composition of zinc, manganese, barium, and aluminum are observed. This new synthesis method paves the way towards the production of magnetosomes for medical applications.
Who reads A Method for Producing Highly Pure Magnetosomes in Large Quantity for Medical Applications Using Magnetospirillum gryphiswaldense MSR-1 Magnetotactic Bacteria Amplified in Minimal Growth Media?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Berny, Clément; Le Fà̈vre, Raphael; Guyot, François; Blondeau, Karine; Guizonne, Christine; Rousseau, Emilie; Bayan, Nicolas; Alphandéry, Edouard
- Publisher
- Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2013- (ISSN 2296-4185)
- Published
- 2020
- Language
- EN
- Field
- Engineering (Physical Sciences)