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At‐line Raman Spectroscopy and Design of Experiments for Robust Monitoring and Control of Miniature Bioreactor Cultures by Ruth C. Rowland‐Jones; Colin Jaques is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is At‐line Raman Spectroscopy and Design of Experiments for Robust Monitoring and Control of Miniature Bioreactor Cultures about?

The biopharmaceutical industry is moving toward a more quality by design (QbD) approach that seeks to increase product and process understanding and process control. Miniature bioreactor systems offer a high‐throughput method enabling the assessment of numerous process variables in a controlled environment. However, the number of off/at‐line samples that can be taken is restricted due to the small working volume of each vessel. This limitation may be resolved through the use of Raman spectroscopy due to its ability to obtain multianalyte data from small sample volumes fast. It can, however, be challenging to implement this technique for this application due to the complexity of the sample matrix and that analytes are often present in low concentration. Here, we present a design of experiments (DOE) approach to generate samples for calibrating robust multivariate predictive models measuring glucose, lactate, ammonium, viable cell concentration (VCC) and product concentration, for unclarified cell culture that improves the daily monitoring of each miniature bioreactor vessel. Furthermore, we demonstrate how the output of the glucose and VCC models can be used to control the glucose a

Who reads At‐line Raman Spectroscopy and Design of Experiments for Robust Monitoring and Control of Miniature Bioreactor Cultures?

Author

Ruth C. Rowland‐Jones; Colin Jaques

Publisher

American Institute of Chemical Engineers; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley; American Chemical Society (ACS) (ISSN 8756-7938)

Published

2018

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)