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Accumulation of Protein Carbonyls Within Cerebellar Astrocytes in Murine Experimental Autoimmune Encephalomyelitis by Jianzheng Zheng; Oscar A. Bizzozero is a Neuroscience article available to read on EtoBox.

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## Abstract Recent work from our laboratory has implicated protein carbonylation in the pathophysiology of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). The present study was designed to determine the changes in protein carbonylation during disease progression and to identify the target cells and modified proteins in the cerebellum of EAE animals, prepared by active immunization of C57/BL6 mice with MOG~35–55~ peptide. In this model, protein carbonylation was maximal at the peak of the disease (acute phase), to decrease thereafter (chronic phase). Double‐immunofluorescence microscopy of affected cerebella showed that carbonyls accumulate in white matter astrocytes and to a lesser extent in microglia/macrophages, in both the acute and the chronic phase. Surprisingly, T cells, oligodendrocytes, and neurons were barely stained. By 2D oxyblot and mass spectrometry, β‐actin, β‐tubulin, GFAP, and HSC‐71 were identified as the major targets of carbonylation throughout the disease. Using a pull‐down/Western blot method, we found a significant increase in the proportion of carbonylated β‐actin, β‐tubulin, and GFAP in the chronic phase but not in the acute phas

Who reads Accumulation of Protein Carbonyls Within Cerebellar Astrocytes in Murine Experimental Autoimmune Encephalomyelitis?

Author

Jianzheng Zheng; Oscar A. Bizzozero

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2010

Language

EN

Field

Neuroscience (Life Sciences)