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Can I read Myocardial Infarction and Functional Outcome Assessment in Pigs on EtoBox?
Myocardial Infarction and Functional Outcome Assessment in Pigs by Koudstaal, Stefan; Jansen of Lorkeers, Sanne; Gho, Johannes M.I.H.; van Hout, Gerardus P.J; Jansen, Marlijn S.; Gründeman, Paul F.; Pasterkamp, Gerard; Doevendans, Pieter A.; Hoefer, Imo E.; Chamuleau, Steven A.J. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Myocardial Infarction and Functional Outcome Assessment in Pigs about?
Introduction of newly discovered cardiovascular therapeutics into first-in-man trials depends on a strictly regulated ethical and legal roadmap. One important prerequisite is a good understanding of all safety and efficacy aspects obtained in a large animal model that validly reflect the human scenario of myocardial infarction (MI). Pigs are widely used in this regard since their cardiac size, hemodynamics, and coronary anatomy are close to that of humans. Here, we present an effective protocol for using the porcine MI model using a closed-chest coronary balloon occlusion of the left anterior descending artery (LAD), followed by reperfusion. This approach is based on 90 min of myocardial ischemia, inducing large left ventricle infarction of the anterior, septal and inferoseptal walls. Furthermore, we present protocols for various measures of outcome that provide a wide range of information on the heart, such as cardiac systolic and diastolic function, hemodynamics, coronary flow velocity, microvascular resistance, and infarct size. This protocol can be easily tailored to meet study specific requirements for the validation of novel cardioregenerative biologics at different stages (_
Who reads Myocardial Infarction and Functional Outcome Assessment in Pigs?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Koudstaal, Stefan; Jansen of Lorkeers, Sanne; Gho, Johannes M.I.H.; van Hout, Gerardus P.J; Jansen, Marlijn S.; Gründeman, Paul F.; Pasterkamp, Gerard; Doevendans, Pieter A.; Hoefer, Imo E.; Chamuleau, Steven A.J.
- Publisher
- MyJoVE Corporation (ISSN 1940-087X)
- Published
- 2014
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)