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Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology by Zeng Xiaobo; Wang haiying; He Linyu; Lin Yongcheng; Li Zhongtao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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To optimize the production of novel ketal compounds and allenic ethers from a mangrove fungus Xylaria sp. 2508, optimization of the culture medium was carried out using a two-step approach. A quick identification of a suitable source of carbon and nitrogen was obtained by a simple screening experiment, which was followed by an application of response surface methodology (RSM) for further optimization design. A second order model was found to fit the eucalyptene A production. Response surface analysis revealed that the optimum values of the tested variables for the production of eucalyptene A were: glucose 2.86% (w/v), peptone 0.78% (w/v) and yeast extract 0.35% (w/v). A production of 4.89 Â 10 À7 g ml À1 for eucalyptene A, which was close to the predicted response, was observed in verification experiment. The production of xyloketal A was 2.20 Â 10 À7 g ml À1 . In comparison to their production of basal medium, 1.53-and 1.32-fold increase had been obtained, respectively. The 3-D response surface curves implied that high yeast extract concentration repressed the growth of Xylaria sp.

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Author

Zeng Xiaobo; Wang haiying; He Linyu; Lin Yongcheng; Li Zhongtao

Publisher

Elsevier Science; Elsevier ; Elsevier Applied Science; Elsevier BV (ISSN 1873-3298)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)