Can I read Free Ia E? chain expression in the E ? + :E ? ? : recombinant strain A.TFR5 on EtoBox?

Free Ia E? chain expression in the E ? + :E ? ? : recombinant strain A.TFR5 by Ann B. Begovich; Patricia P. Jones is a Immunology and Microbiology article available to read on EtoBox.

What is Free Ia E? chain expression in the E ? + :E ? ? : recombinant strain A.TFR5 about?

Molecular and biochemical techniques have been used to explore the reasons behind low E~ chain expression in the E~+E~ I-region recombinant strain, A.TFR5. A.TFR5 (AIE k, ap5), a recombinant between A.CA (AlE ~) and A.TL (AkEg), carries the E k subregion. Previous results have shown that it + + expresses the E, chain, but at reduced levels relative to E~ E B strains. No E~ chains were detected, which is consistent with the A.TFR5E~ gene being derived from the A.CA parent, which carries the null E~ allele. In this paper, the defect in E~-chain expression is explored. Restriction fragment length polymorphism analysis has localized the recombination event in A.TFR5 approximately 30 kb upstream of E~, in the region of the large intervening sequence of Ep. Northern blot analysis of total RNA from A.TFR5 shows normal amounts of the E~ message, but no E~ message. Two-dimensional gel analysis of 15 min pulselabeled A.TFR5, A.CA, and A.TL E immunoprecipitates shows decreased levels of the intracellular E~ chain in A.TFR5 relative to A.TL. However, analysis of total cell extracts shows normal levels of this protein. A glycoprotein fraction isolated from total cell extracts of 5 h labeled cel

Who reads Free Ia E? chain expression in the E ? + :E ? ? : recombinant strain A.TFR5?

Author

Ann B. Begovich; Patricia P. Jones

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0093-7711)

Published

1985

Language

EN

Field

Immunology and Microbiology (Life Sciences)