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Very low-angle light scattering. A characterization method for high-molecular-weight DNA by Harold I. Levine; Robert J. Fiel; Fred W. Billmeyer Jr. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A very low-angle light-scattering photometer is described with respect to optical features, scattering cell, correction factors, and absolute calibration in the angular range 2'-35". An improved microfiltration apparatus was used to obtain essentially dust-free aqueous solutions for very low-angle light scattering. The instrument was calibrated with silicotungstic acid, an absolute molecular-weight standard, and the calibration was confirmed with the use of several secondary standards. Very low-angle light-scattering measurements were made to determine the weight-average molecular weight M r and z-average radius of gyration Rg,\* of a commercial preparation of calf-thymus DNA. Microfiltration of the solutions allowed measurements down to 6". The value M, = 20.0 X lo6 obtained by extrapolating 6"-9O data to 0' is more than three times that from 30'-75" data (6.38 X lo6) but -50% smaller than that from 10-35" data (23.7 X lo6). The experimental errors in M r and Rg,L are estimated to be f8% and 41496, respectively. Combined 6"-75" data from two photometers fit well a theoretical scattering curve for a model wormlike coil of the same M, as the DNA sample.

Who reads Very low-angle light scattering. A characterization method for high-molecular-weight DNA?

Author

Harold I. Levine; Robert J. Fiel; Fred W. Billmeyer Jr.

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)

Published

1976

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)