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Characterization of RCI-1, a chloroplastic rice lipoxygenase whose synthesis is induced by chemical plant resistance activators by Ulrich Schaffrath; Frauke Zabbai; Robert Dudler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A full‐length lipoxygenase cDNA (__RCI‐1__) has been cloned from rice (__Oryza sativa__) whose corresponding transcripts accumulate in response to treatment of the plants with chemical inducers of acquired resistance such as benzo(1,2,3)thiadiazole‐7‐carbothioic acid __S__‐methyl ester (BTH), 2,6‐dichloroisonicotinic acid (INA), and probenazol. In contrast, __RCI‐1__ transcript levels did not increase after inoculation with compatible and incompatible races of the rice blast fungus __Magnaporthe grisea__ and the nonhost pathogen __Pseudomonas syringae__ pv. __syringae__. __RCI‐1__ transcript levels also increased after exogenous application of jasmonic acid, but not upon wounding. Dose–response and time course experiments revealed a similar pattern of transcript accumulation and lipoxygenase activity in BTH‐treated rice leaves. Enzymatic analysis of recombinant RCI‐1 protein produced in __Escherichia coli__ revealed that 13‐hydroperoxy‐octadecanoic acids were the predominant reaction products when either linoleic or linolenic acid used as a substrate. The RCI‐1 sequence features a putative chloroplast targeting sequence at its N‐terminus. Indeed, a protein consisting of the putativ

Who reads Characterization of RCI-1, a chloroplastic rice lipoxygenase whose synthesis is induced by chemical plant resistance activators?

Author

Ulrich Schaffrath; Frauke Zabbai; Robert Dudler

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Physical Sciences)