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Increased 15-Lipoxygenase-1 Expression in Chondrocytes Contributes to the Pathogenesis of Osteoarthritis by Kaizhe Chen; Yufei Yan; Changwei Li; Jun Yuan; Fei Wang; Ping Huang; Niandong Qian; Jin Qi; Hanbing Zhou; Qi Zhou; Lianfu Deng; Chuan He; Lei Guo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Increased 15-Lipoxygenase-1 Expression in Chondrocytes Contributes to the Pathogenesis of Osteoarthritis about?

Lipoxygenase-1 (15-LO-1) is involved in many pathological processes. The purpose of this study was to determine the potential role of 15-LO-1 in osteoarthritis (OA). The levels of 15-LO-1 expression were measured by western blotting and quantitative realtime PCR in articular cartilage from the OA rat models and OA patients. To further investigate the effects of 15-LO-1 on chondrocyte functions, such as extracellular matrix (ECM) secretion, the release of matrix-degrading enzymes, the production of reactive oxygen species (ROS), cell proliferation and apoptosis, we decreased or increased 15-LO-1 expression in chondrocytes by means of transfecting with siRNA targeting 15-LO-1 and plasmid encoding 15-LO-1, respectively. The results showed that 15-LO-1 expression was obviously increased in articular cartilage from OA rats and OA patients. It was also found that many factor-related OA, such as mechanical loading, ROS, SNP and inflammatory factor, significantly promoted 15-LO-1 expression and activity in chondrocytes. Silencing 15-LO-1 was able to markedly alleviate mechanical loading-induced cartilage ECM secretion, cartilagedegrading enzyme secretion and ROS production. Overexpression

Who reads Increased 15-Lipoxygenase-1 Expression in Chondrocytes Contributes to the Pathogenesis of Osteoarthritis?

Author

Kaizhe Chen; Yufei Yan; Changwei Li; Jun Yuan; Fei Wang; Ping Huang; Niandong Qian; Jin Qi; Hanbing Zhou; Qi Zhou; Lianfu Deng; Chuan He; Lei Guo

Publisher

Nature Publishing Group; Springer Science and Business Media LLC; London: Nature Publishing Group, 2001- (ISSN 2041-4889)

Published

2017

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)