Opening book details…
Can I read The Use of Regenerable, Affinity Ligand-based Surfaces for Immunosensor Applications on EtoBox?
The Use of Regenerable, Affinity Ligand-based Surfaces for Immunosensor Applications by John Quinn; Pritesh Patel; Brian Fitzpatrick; Bernadette Manning; Paul Dillon; Stephen Daly; Richard O'Kennedy; Marcos Alcocer; Heather Lee; Michael Morgan; Kenny Lang is a Engineering article available to read on EtoBox.
What is The Use of Regenerable, Affinity Ligand-based Surfaces for Immunosensor Applications about?
The regeneration of antibody-binding surfaces is of major importance for re-usable sensor formats such as required for direct 'real-time' biosensing technologies and is often difficult to achieve. Antibodies commonly bind the antigen with high avidity and may themselves be sensitive to regeneration conditions. The interaction of polyclonal anti-chlorpyriphos antibody with an immobilised chlorpyriphos-ovalbumin (chlor-oval) conjugate and the interaction of soluble recombinant CD4 with covalently immobilised anti-CD4 IgG are presented in order to highlight these difficulties. Affinity-capture is suggested as an alternative format as it facilitates surface regeneration, directed immobilisation and the attainment of interaction progress curves that conform to the ideal pseudo-first-order kinetic interaction model. Protein A, protein G and polyclonal anti-mouse Fc-coated surfaces were used to observe the interaction of captured anti-GST monoclonal antibody with glutathione-s-transferase (GST). It was shown that a protein A affinity-capture surface produced ideal interaction progress curves while both protein G and polyclonal anti-mouse Fc resulted in systemic deviations.
Who reads The Use of Regenerable, Affinity Ligand-based Surfaces for Immunosensor Applications?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- John Quinn; Pritesh Patel; Brian Fitzpatrick; Bernadette Manning; Paul Dillon; Stephen Daly; Richard O'Kennedy; Marcos Alcocer; Heather Lee; Michael Morgan; Kenny Lang
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 1999
- Language
- EN
- Field
- Engineering (Physical Sciences)