Can I read Expression of the Brain-type Glucose Transporter Is Restricted to Brain and Neuronal Cells in Mice on EtoBox?

Expression of the Brain-type Glucose Transporter Is Restricted to Brain and Neuronal Cells in Mice by G. W. Gould; A. M. Brant; B. B. Kahn; P. R. Shepherd; S. C. McCoid; E. M. Gibbs is a Medicine article available to read on EtoBox.

What is Expression of the Brain-type Glucose Transporter Is Restricted to Brain and Neuronal Cells in Mice about?

Northern blot analysis of human tissues has demonstrated the expression of the brain-type glucose transporter isoform (GLUT 3) in liver, muscle and fat, raising the possibility that this transporter isoform may play a role in the regulation of glucose disposal in these tissues in response to insulin. We have raised an anti-peptide antibody against the C-terminal 13 amino acids of the murine homologue of this transporter isoform, and determined its tissue distribution in mouse tissues and murine-derived cell lines. The antibodies recoguise a glycoprotein of about 50 kilodaltons, expressed at high levels in murine brain. In contrast to human tissues, the expression of GLUT 3 in mice is restricted to the brain, and no immunoreactivity was observed in either liver, fat or muscle membranes, or in murine 3T3-L1 fibroblasts or adipocytes. In contrast, high levels of expression of this isoform were observed in the NG 108 neuroblastoma x glioma cell line, a hybrid cell derived from rat glioma and mouse neuroblastoma cells. Taken together, these data suggest that the expression of GLUT 3 in rodents is restricted to non-insulin responsive neuronal cells and hence it is likely that the factors

Who reads Expression of the Brain-type Glucose Transporter Is Restricted to Brain and Neuronal Cells in Mice?

Author

G. W. Gould; A. M. Brant; B. B. Kahn; P. R. Shepherd; S. C. McCoid; E. M. Gibbs

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0012-186X)

Published

1992

Language

EN

Field

Medicine (Health Sciences)