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Fabrication of High-aspect-ratio Amorphous Perfluorinated Polymer Structure for Total Internal Reflection Fluorescence Microscopy by Asahi Nakahara; Yoshitaka Shirasaki; Kentaro Kawai; Osamu Ohara; Jun Mizuno; Shuichi Shoji is a Engineering article available to read on EtoBox.
What is Fabrication of High-aspect-ratio Amorphous Perfluorinated Polymer Structure for Total Internal Reflection Fluorescence Microscopy about?
A method to fabricate microstructures that are ideal for optical applications was developed. This method enabled structures with a high aspect ratio to be fabricated from an amorphous perfluorinated polymer, CYTOP, which has an identical refractive index to that of water. Using the method, a microwell array device was developed for the purpose of high-throughput protein quantification. Evaluation of the optical properties of the array using total internal reflection fluorescence (TIRF) microscopy demonstrated that the device did not interfere with TIRF imaging at the bottom of each microwell. This method can expand the possible applications of biophotonic devices as well as TIRF imaging.
Who reads Fabrication of High-aspect-ratio Amorphous Perfluorinated Polymer Structure for Total Internal Reflection Fluorescence Microscopy?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Asahi Nakahara; Yoshitaka Shirasaki; Kentaro Kawai; Osamu Ohara; Jun Mizuno; Shuichi Shoji
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0167-9317)
- Published
- 2011
- Language
- EN
- Field
- Engineering (Physical Sciences)