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Water-soluble Proteins of Mature Barley Endosperm: Genetic Control, Polymorphism, and Linkage with β-Amylase and Spring/winter Habit by B. P. Forster; D. M. Thompson; J. Watters; W. Powell is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Water-soluble Proteins of Mature Barley Endosperm: Genetic Control, Polymorphism, and Linkage with β-Amylase and Spring/winter Habit about?

Water-soluble proteins (WSP-2 and WSP-3) and β-amylase (β-AMY-1) were extracted from mature endosperms of 44 spring and 39 winter barley genotypes. The protein and enzyme isoforms were separated in isoelectric focusing gels with a pH gradient of 4-6.5. The Wsp-3 and β-Amy-1 loci were located to chromosomes 4H using the wheat/barley chromosome addition lines. Segregation analysis of F2 and doubled haploid populations showed Wsp-2 and β-Amy-1 to be tightly linked, with a map distance of 11 cMorgans. Isoforms of WSP-2 possessed similar pIs to that of WSP-3 and overlapping bands were observed in the gels. These bands segregated independently in F2 and doubled haploid populations, implying two unlinked genes. All three loci were found to be polymorphic: two alleles were detected at the Wsp-2 locus, three at Wsp-3 and two at β-Amy-1. The frequency of alleles at all three loci was found to be different in winter and spring genotypes. Spring genotypes possessed a wider range of phenotypes than winter genotypes. Spring and winter genotypes could be distinguished on the basis of WSP-3 and β- AMY-1 phenotypes. The linkage between Wsp-3 and β-Amy-1 loci and genes controlling spring/winter habi

Who reads Water-soluble Proteins of Mature Barley Endosperm: Genetic Control, Polymorphism, and Linkage with β-Amylase and Spring/winter Habit?

Author

B. P. Forster; D. M. Thompson; J. Watters; W. Powell

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 0040-5752)

Published

1991

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)