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The barley powdery mildew protein kinase C gene, pkc 1 and pkc ‐like gene, are differentially expressed during morphogenesis by Ziguo Zhang; Gemma Priddey; Sarah Jane Gurr is a Agricultural and Biological Sciences article available to read on EtoBox.

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## summary Protein kinase C agonist assays revealed the phorbol ester, phorbol 12‐myristate 13‐acetate, invoked germling morphogenesis and enhanced PKC activity in __Blumeria graminis__. No antagonist of mildew PKC activity was found but the data fuelled a hunt for powdery mildew __pkc__ genes. Oligonucleotides, designed on the basis of conserved ATP‐binding and kinase domains within the catalytic core of eukaryotic protein kinase proteins, were used as primers to amplify chromosomal and cDNA fragments from the barley powdery mildew fungus __graminis__. Three kinase gene fragments were isolated (__pkc__1, __pkc__‐like and c__pka__) and the full length genomic sequences of the mildew __pkc__ and __pkc‐__like genes were determined by ‘step down’ PCR. RT‐PCR transcript profiles showed the three genes to be differentially regulated during germling morphogenesis.

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Author

Ziguo Zhang; Gemma Priddey; Sarah Jane Gurr

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1464-6722)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)