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Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp by Jin-Xia Peng; Jian-Li Xie; Li Zhou; Yun-Han Hong; Jian-Fang Gui is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract To investigate germline development and germ cell specification, we identified a __Dazl__ homolog (__CagDazl__) from gynogenetic gibel carp (__Carassius auratus gibelio__). Its cDNA sequence and BAC clone sequence analyses revealed the genomic organization conservation and conserved synteny of the __Dazl__ family members and their neighborhood genes among vertebrates, especially in fish. Moreover, a polyclonal antibody specific to __Cag__Dazl was produced and used to examine its expression and distribution throughout germline development at protein level. Firstly, ovary‐specific expression pattern of __CagDazl__ was confirmed in adult tissues by RT‐PCR and Western blot. In addition, in situ hybridization and immunofluorescence localization demonstrated its specific expression in germ cells, and both its transcript and protein were localized to germ plasm. Then, co‐localization of __Cag__Dazl and mitochondrial cloud was found, confirming that __CagDazl__ transcript and its protein are germ plasm component and move via METRO pathway during oogenesis. Furthermore, the __Cag__Dazl is abundant and continuous throughout germline development and germ cell specification includi

Who reads Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp?

Author

Jin-Xia Peng; Jian-Li Xie; Li Zhou; Yun-Han Hong; Jian-Fang Gui

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1552-5007)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)