Can I read Visualized investigation of yeast transformation induced with Li+ and polyethylene glycol on EtoBox?

Visualized investigation of yeast transformation induced with Li+ and polyethylene glycol by Ping Chen; Hui-Hui Liu; Ran Cui; Zhi-Ling Zhang; Dai-Wen Pang; Zhi-Xiong Xie; Hu-Zhi Zheng; Zhe-Xue Lu; Hua Tong is a Chemistry article available to read on EtoBox.

What is Visualized investigation of yeast transformation induced with Li+ and polyethylene glycol about?

The effects of Li(+) and polyethylene glycol (PEG) on the genetic transformation of Saccharomyces cerevisiae were investigated by using fluorescence microscopy (FM) to visualize the binding of plasmid DNA labeled with YOYO-1 to the surface of yeast cells, scanning electron microscopy (SEM) and atomic force microscopy (AFM) to image the change in surface topography of yeast cells, coupled with transformation frequency experiments. The results showed that under the same conditions, the transformation frequencies of yeast protoplasts were much higher than those of intact yeast cells. PEG was absolutely required for the binding of DNA to the surface of intact yeast cells or yeast protoplasts, and had no effect on the surface topography of intact yeast cells or yeast protoplasts. In the presence of PEG, Li(+) could greatly enhance the binding of plasmid DNA to the surface of intact yeast cells, increase their transformation frequency, and affect their surface topography. On the other hand, no effect on the DNA binding to the surface of protoplasts and no increase in the number of transformants and no surface topography changes were found upon the treatment with Li(+) to protoplasts. In

Who reads Visualized investigation of yeast transformation induced with Li+ and polyethylene glycol?

Author

Ping Chen; Hui-Hui Liu; Ran Cui; Zhi-Ling Zhang; Dai-Wen Pang; Zhi-Xiong Xie; Hu-Zhi Zheng; Zhe-Xue Lu; Hua Tong

Publisher

Elsevier BV

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)