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A Robust Protein Host for Anchoring Chelating Ligands and Organocatalysts by Manfred T. Reetz; Martin Rentzsch; Andreas Pletsch; Andreas Taglieber; Frank Hollmann; Régis J. G. Mondière; Norbert Dickmann; Birte Höcker; Simona Cerrone; Michaela C. Haeger; Reinhard Sterner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A Robust Protein Host for Anchoring Chelating Ligands and Organocatalysts about?
## Abstract In order to put the previously proposed concept of directed evolution of hybrid catalysts (proteins that harbor synthetic transition‐metal catalysts or organocatalysts) into practice, several prerequisites must be met. The availability of a robust host protein that can be expressed in sufficiently large amounts, and that can be purified in a simple manner is crucial. The thermostable enzyme tHisF from __Thermotoga maritima__, which constitutes the synthase subunit of a bi‐enzyme complex that is instrumental in the biosynthesis of histidine, fulfills these requirements. In the present study, fermentation has been miniaturized and parallelized, as has purification of the protein by simple heat treatment. Several mutants with strategically placed cysteines for subsequent bioconjugation have been produced. One of the tHisF mutants, Cys9Ala/Asp11Cys, was subjected to bioconjugation by the introduction of a variety of ligands for potential metal ligation, of a ligand/metal moiety, and of several organocatalytic entities that comprise a flavin or thiazolium salts. Characterization by mass spectrometry and tryptic digestion was achieved. As a result of this study, a platform fo
Who reads A Robust Protein Host for Anchoring Chelating Ligands and Organocatalysts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Manfred T. Reetz; Martin Rentzsch; Andreas Pletsch; Andreas Taglieber; Frank Hollmann; Régis J. G. Mondière; Norbert Dickmann; Birte Höcker; Simona Cerrone; Michaela C. Haeger; Reinhard Sterner
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1439-4227)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)