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Flow-stream Waveguide for Collection of Perpendicular Light Scatter in Flow Cytometry by Raymond Mariella Jr.; Gerrit van den Engh; Donald Masquelier; Gerald Eveleth is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We report a new physical configuration for the detection of perpendicular light scatter or fluorescence in flow cytometry when using a fluid stream in air. "his configuration increases the signal-tonoise ratio and narrows the coeEicient of variation for uniformly sized latex spheres when compared to using a microscope objective to collect such light. The new technique views the scattered light that is trapped within the optical waveguide that is M ~Urally formed by the flow stream in air. One efficient and simple way to detect the light trapped within this optical waveguide is to place one end of a fiber optic, with a conically polished tip and sufficiently large-core diameter, directly into the flow stream and to place an optical detector at the fiber's other end. For perpendicular light scatter, the flowstream waveguide achieves high collection efficiency (NA = 0.88) as well as high efficiency of optical throughput due to lack of surfaces between the light scatterem and the detector. We obtained 10fold higher signals with this technique than with a long-working-distance microscope objective. The flow-stream waveguide is also much easier to align than traditional microscope-lens-b

Who reads Flow-stream Waveguide for Collection of Perpendicular Light Scatter in Flow Cytometry?

Author

Raymond Mariella Jr.; Gerrit van den Engh; Donald Masquelier; Gerald Eveleth

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)