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Subtle decreases in DNA methylation and gene expression at the mouse Igf2 locus following prenatal alcohol exposure: effects of a methyl-supplemented diet by Chris Downing; Thomas E. Johnson; Colin Larson; Tatiana I. Leakey; Rachel N. Siegfried; Tonya M. Rafferty; Craig A. Cooney is a Medicine article available to read on EtoBox.
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C57BL/6J (B6) mice are susceptible to in utero growth retardation and a number of morphological malformations following prenatal alcohol exposure, while DBA/2J (D2) mice are relatively resistant. We have previously shown that genomic imprinting may play a role in differential sensitivity between B6 and D2. The best-characterized mechanism mediating genomic imprinting is differential DNA methylation. In the present study we examined DNA methylation and gene expression, in both embryonic and placental tissue, at the mouse Igf2 locus following in utero ethanol exposure. We also examined the effects of a methyl-supplemented diet on methylation and ethanol teratogenesis. In embryos from susceptible B6 mice, we found small decreases in DNA methylation at four CpG sites in one of the differentially methylated regions of the Igf2 locus; only one of the four sites showed a statistically significant decrease. We observed no significant decreases in methylation in placentae. All Igf2 transcripts showed approximately 1.5-fold decreases following intrauterine alcohol exposure. Placing dams on a methyl-supplemented diet before pregnancy and throughout gestation brought methylation back up to con
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- Author
- Chris Downing; Thomas E. Johnson; Colin Larson; Tatiana I. Leakey; Rachel N. Siegfried; Tonya M. Rafferty; Craig A. Cooney
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0741-8329)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)