About this Biochemistry, Genetics and Molecular Biology article
Dexamethasone-dependent Versus -Independent Markers of Epithelial to Mesenchymal Transition in Primary Hepatocytes by Patricio Godoy; Sumathi Lakkapamu; Markus Schug; Alexander Bauer; Joanna D. Stewart; Essam Bedawi; Seddik Hammad; Jakia Amin; Rosemarie Marchan; Wiebke Schormann; Lindsey Maccoux; Iris von Recklinghausen; Raymond Reif; Jan G. Hengstler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
## Abstract Recently, epithelial to mesenchymal transition (EMT) has been shown to represent a feature of dedifferentiating hepatocytes __in vitro__. Three-dimensional soft collagen gels can antagonize but not completely abolish this effect. Hormonal additives to culture media are known to maintain differentiated hepatocyte functions. Therefore, we studied whether insulin and dexamethasone antagonize EMT in cultured hepatocytes. Both hormones antagonized but not completely abolished certain morphological features of EMT. Dexamethasone antagonized acquisition of fibroblastoid shape, whereas insulin favored bile canaliculi formation. In a subsequent step, we analyzed expression of a battery of EMT-related genes. Of all markers tested, vimentin and snail-1 correlated best with morphological features of EMT. Interestingly, dexamethasone reduced expression levels of both vimentin and snail-1, whereas the influence of insulin was less pronounced. An important result of this study is that 12 out of 17 analyzed EMT markers were transcriptionally influenced by dexamethasone (vimentin, snail-1, snail-2, HNF4α, Twist-1, ZEB2, fibronectin, occludin, MMP14, claudin-1, cytokeratin-8, and cytoker
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- Author
- Patricio Godoy; Sumathi Lakkapamu; Markus Schug; Alexander Bauer; Joanna D. Stewart; Essam Bedawi; Seddik Hammad; Jakia Amin; Rosemarie Marchan; Wiebke Schormann; Lindsey Maccoux; Iris von Recklinghausen; Raymond Reif; Jan G. Hengstler
- Publisher
- Walter de Gruyter GmbH
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)