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Can I read A Dual Immunocytochemical Assay for Oestrogen and Epidermal Growth Factor Receptors in Tumour Cell Lines on EtoBox?
A Dual Immunocytochemical Assay for Oestrogen and Epidermal Growth Factor Receptors in Tumour Cell Lines by A. K. Sharma; K. Horgan; R. A. McClelland; A. G. Douglas-Jones; T. Van Agthoven; L. C. J. Dorssers; R. I. Nicholson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A Dual Immunocytochemical Assay for Oestrogen and Epidermal Growth Factor Receptors in Tumour Cell Lines about?
A new dual immunocytochemical assay for oestrogen receptor (ER) and epidermal growth factor receptor (EGFR) has been developed. It has been tested in a variety of conditions using cell culture lines and the results correlate well with those obtained from single immunocytochemical assays. MCF-7 and A43I cells and a mixture of the two types of cell were assessed immunocytochemically for ER and EGFR. ER showed immunopositivity of 30% in MCF-7 cells, I0% in the mixture and 0% in A43I cells. EGFR demonstrated immunopositivity of 0% in MCF-7 cells, 70% in the mixture and 100% in A431 cells. Dual immunocytochemical assays using anti-ER followed by anti-EGFR monoclonal antibodies on single histological sections showed similar reactivity to the single assays. Three staining patterns were seen in the mixture: ER+/EGFR-(MCF-7 cells), ER-/EGFR-(MCF-7 cells) and ER-/EGFR+ (A431 cells). ZR-75-I and MDA-MB-231 cells and their retrovirally transfected counterparts ZR/HERc and MDA/HEGO cells were then analysed. The dual assay revealed the fourth phenotype (ER+/EGFR+)in 40% of ZR/HERc cells and in 10% of MDA/HEGO cells. This is the first description of a dual immunocytochemical assay system for ER a
Who reads A Dual Immunocytochemical Assay for Oestrogen and Epidermal Growth Factor Receptors in Tumour Cell Lines?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- A. K. Sharma; K. Horgan; R. A. McClelland; A. G. Douglas-Jones; T. Van Agthoven; L. C. J. Dorssers; R. I. Nicholson
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0018-2214)
- Published
- 1994
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)