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Hypoxia and Porphyromonas gingivalis-lipopolysaccharide synergistically induce NLRP3 inflammasome activation in human gingival fibroblasts by Kai Yang; Shuo Xu; Hongmei Zhao; Lingshuang Liu; Xiaofang Lv; Fang Hu; Lei Wang; Qiuxia Ji is a Immunology and Microbiology article available to read on EtoBox.
What is Hypoxia and Porphyromonas gingivalis-lipopolysaccharide synergistically induce NLRP3 inflammasome activation in human gingival fibroblasts about?
## Objective: To investigate the effects of hypoxia and porphyromonas gingivalis- lipopolysaccharide (p. gingivalis-lps) on activation of the nacht leucine-rich repeat protein 3 (nlrp3) inflammasome in human gingival fibroblasts (hgfs). ## Design: Periodontitis was optimally simulated using a hypoxic concentration of 1%. hgfs were stimulated using p. gingivalis-lps (1.0 μg/ml) in normoxia and hypoxia for 3 h and 6 h, respectively. the expression levels of genes and proteins of hypoxia-inducible factor-1α (hif-1α), interleukin-1β, gasdermin d (gsdmd) and the nlrp3 inflammasome, including nlrp3, apoptosis-associated speck-like protein containing card (asc), caspase-1 and its activated forms, were measured using quantitative real-time polymerase chain reaction and western blot. elisa was used to detect and determine levels of the inflammatory factor interleukin-1β in cell supernatants. lactate dehydrogenase (ldh) release assay, caspase-1 activity assay and hoechst 33342/propidium iodide (pi) staining were performed to further verify the presence of pyroptosis. ## Results: The nlrp3 inflammasome (i.e., nlrp3, asc, caspase-1) was not affected by individual stimulation using p. gingivali
Who reads Hypoxia and Porphyromonas gingivalis-lipopolysaccharide synergistically induce NLRP3 inflammasome activation in human gingival fibroblasts?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Kai Yang; Shuo Xu; Hongmei Zhao; Lingshuang Liu; Xiaofang Lv; Fang Hu; Lei Wang; Qiuxia Ji
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)