About this document
Illumina P5 and P7 Sequence Guidelines by lsfnano is a document available to read on EtoBox.
Illumina library structure requires full-length P5 and P7 sequences for HiSeq and MiSeq sequencing. Custom sequencing primers can be used on Read1 for both instruments, but Read2 custom primers are limited to MiSeq unless specific conditions are met. Proper sequence diversity is crucial for library performance, particularly on MiSeq, and may require the addition of balancer DNA if sequences are not base-balanced.
- Author
- lsfnano
- Language
- EN