Can I read Illumina P5 and P7 Sequence Guidelines on EtoBox?

Illumina P5 and P7 Sequence Guidelines by lsfnano is a document available to read on EtoBox.

What is Illumina P5 and P7 Sequence Guidelines about?

Illumina library structure requires full-length P5 and P7 sequences for HiSeq and MiSeq sequencing. Custom sequencing primers can be used on Read1 for both instruments, but Read2 custom primers are limited to MiSeq unless specific conditions are met. Proper sequence diversity is crucial for library performance, particularly on MiSeq, and may require the addition of balancer DNA if sequences are not base-balanced.

Author

lsfnano

Language

EN