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Three-dimensional Reconstruction of Tetraploid↔diploid Chimaeric Mouse Blastocysts by CLARE A. EVERETT; MARGARET H. STARK; JOHN D. WEST; DUNCAN DAVIDSON; RICHARD A. BALDOCK is a Earth and Planetary Sciences article available to read on EtoBox.

What is Three-dimensional Reconstruction of Tetraploid↔diploid Chimaeric Mouse Blastocysts about?

Studies of tetraploid↔diploid (4n↔2n) mouse chimaeras have demonstrated unequal contributions of 4n cells to different tissues of the midgestation conceptus. Such a pattern has also been reported in chimaeras as early as E3.5d, which show an enhanced contribution of 4n cells to the mural trophectoderm (Everett & West, 1996). In this study, sectioned 4n↔2n and 2n↔2n control chimaeric blastocysts were digitised and reconstructed in 3 dimensions (3‐D). The 3‐D images revealed only limited mixing of cells from the 2 contributing embryos of individual blastocysts in both chimaera groups. Consequently, the distribution pattern of the 2 cell types was dependent on the spatial relationship between the orientation of the blastocyst and the boundary between the 2 clusters of cells. The distribution patterns observed were not strikingly different for 4n↔2n and 2n↔2n chimaeras, each showing some transgenic positive cell contribution in all 3 identifiable developmental lineages. It was notable, however, that in all 4n↔2n blastocysts at least some 4n cells were located adjacent to the blastocyst cavity. Such a consistent pattern was not evident in 2n↔2n chimaeras. This study has demonstrated the

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Author

CLARE A. EVERETT; MARGARET H. STARK; JOHN D. WEST; DUNCAN DAVIDSON; RICHARD A. BALDOCK

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0021-8782)

Published

2000

Language

EN

Field

Earth and Planetary Sciences (Physical Sciences)