About this document
SDS-PAGE Protocol for Protein Analysis by Gowtham Manimekalai is a document available to read on EtoBox.
This document provides instructions for performing SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) to separate proteins by size. It describes how to assemble the gel apparatus and pour the stacking and running gels, load samples, run the electrophoresis, and stain the gel to visualize protein bands. Stock solutions like acrylamide, buffers, and staining solutions are also defined to perform SDS-PAGE.
- Author
- Gowtham Manimekalai
- Language
- EN