Can I read Immunological screening for tumor cells in serous body fluids has added value with the CELL-DYN Sapphire on EtoBox?

Immunological screening for tumor cells in serous body fluids has added value with the CELL-DYN Sapphire by Jeffrey F.W. Keuren; Johannes J.M.L. Hoffmann; Mathie P.G. Leers is a Medicine article available to read on EtoBox.

What is Immunological screening for tumor cells in serous body fluids has added value with the CELL-DYN Sapphire about?

Background: Conventional cytological examination has limited sensitivity for detecting tumor cells in serous body cavity effusions and therefore, adjuvant techniques are necessary for a reliable diagnosis. Flow cytometry has proven benefit in these circumstances. The aim of our study was to explore the feasibility of CELL-DYN Sapphire, an advanced hematology analyzer with flow cytometric capabilities, for detecting tumor cells in serous body fluids, using CD326 monoclonal antibodies, which are directed against the epithelial marker EpCAM. Methods: One hundred and five serous fluids (39 peritoneal and 66 pleural effusions) were analyzed by the CELL-DYN Sapphire using monoclonal antibody combinations CD3/CD19 and CD45/CD326. Of all samples a cytospin preparation was made and microscopically examined; the pathology findings served as a reference. Results: Using a threshold of 1% CD326+ cells, CELL-DYN Sapphire identified nine out of 12 cases with tumor cells in the serous effusions (sensitivity 75%), whereas routine cytology found eight cases (sensitivity 67%). The combination of immunophenotyping and cytology identified all 12 cases with tumor cells in the effusion fluid (sensitivity

Who reads Immunological screening for tumor cells in serous body fluids has added value with the CELL-DYN Sapphire?

Author

Jeffrey F.W. Keuren; Johannes J.M.L. Hoffmann; Mathie P.G. Leers

Publisher

Walter de Gruyter GmbH

Published

2014

Language

EN

Field

Medicine (Health Sciences)